The objective of the research was a comparative assessment of the infection levels of Nosema spp. in honey bees collected from different areas of the hive. A total of 588 honey bee colonies were sampled in spring (April-May) 2015 and 2016 through the simultaneous collection of dead worker bees from the bottom board of the hive and live bees from peripheral combs. https://www.rawafricaonline.com/